产品描述


The EpiSonic? 2000 Sonication System is the latest sonication-based instrument with integrated cooling for DNA shearing and chromatin shearing applications. This sonicator is designed for simultaneous, optimized processing of 1 to 12 samples at small microliter-level volumes (optimal at 15 μl of volume), making it an ideal solution for next-generation sequencing due to its high shearing consistency and uniformity.

Features At-A-Glance
The EpiSonic? 2000 incorporates Digitally Adaptive Sonocavitation? (DAS) technology to efficiently process DNA and chromatin samples, with the following key features:

  • Simple input and controls
  • Simultaneous sonication of up to 12 samples
  • 10 minutes to shear 12 samples to 300 bp (50 sec/sample)
  • Reproducible and consistent fragments
  • Closed vessel processing
  • Easy installation and maintenance
  • Continuous cooling system
  • 20-month warranty

Key Points
Notable points of the EpiSonic? 2000 include the following:

  • Generates fragments compatible with IlluminaIon Torrent454, and Pacific Biosciences platforms.
  • Generates tight and highly reproducible fragment distributions.
  • Capable of achieving uniform fragment sizes between 150 bps and 20 kbs.
  • Meets "the scientist's requirements" for a low-cost, flexible, higher throughput and easy-to-use design.
  • Eliminates operator-induced variations, improves recovery yield, increases efficiency, and provides results that can be standardized.

High throughput sonication

Fig. 2. Demonstration of consistent fragments achieved with a single run of 12-sample multiplex shearing with the EpiSonic? 2000.


DAS? technology, incorporated into the EpiSonic? 2000, provides the following advantages:

  • Controlled energy delivery
  • Isothermal environment
  • Non-contact, closed vessel processing
  • Enhanced reproducibility
  • Uniform multiplex sample shearing
  • User selectable fragment lengths
  • High sample recovery with no heat damage
  • No cross-contamination, no aerosols, no clean up
  • Micro-scale sample volumes
  • Real-time process monitoring
High throughput sonication

Fig. 1. Setup of the EpiSonic? 2000 Sonication System. 


Specifications

Model Number 2000
Input Voltage 100-120 VAC or 220-240 VAC @ 50/60 Hz
Power Rating 750 Watts
Programmable Timer Up to 10 hours
Operating Frequency 20 kHz
Timer Control Digital
Throughput Capability Up to 12
Chiller Dimensions  7.5"L x 5"W x 7"H (19 cm x 12.7 cm x 17.6 cm)
Generator Dimensions 15.25"L x 8"W x 8.5"H (38.7 cm x 20.3 cm x 21.6 cm)
Sound Enclosure Dimensions 10"L x 10"W x 16.5"H (25.5 cm x 25.5 cm x 42 cm)
Operating Environment Temperature: 39°-95°F (4°-35°C)
Relative Humidity 20-90% (Non Condensing)
For indoor use only
Origin of Manufacture USA
Warranty Period 20 months

Reproducibility at 150 bp shearing Reproducibility at 300 bp shearing Reproducibility at 350 bp shearing

Fig 2

Fig 3

Fig 4

 

Reproducibility at 2 kb shearing

 

Reproducibility at 6 kb shearing

 

Reproducibility at 20 kb shearing

Fig 5 Fig 6 Fig 5

 


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